Guanidine Hydrochloride is a strong denaturant, which is used in studies that are physiochemical in nature, focusing on protein folding. It is the hydrochloride salt of the compound Guanidine. It is also known as Guanidine Chloride, and it is abbreviated as GdmCL, GuHCI, or GndCI.
The compound GdmCI is very soluble in both ethanol and water. The melting point is 360 degrees Fahrenheit, or 182.3 degrees Celsius. It has a density of 1.354 g/cm3 at 20 degrees Celsius. The appearance of the compound is in crystal form. When refolding enzymes or proteins back into an active form, it can be used as the very first step of the process.
When working with this compound, certain safety precautions should be taken. Such precautions include wearing gloves, a face shield, an eye shield, and a dust mask, type N95. The compound is one of the strongest denaturants available, and it has chaotropic properties.